If so today you're going to learn.
Get Full Essay Get access to this section to get all help you need with your essay and educational issues. This was done by using the independent variable of alcohol to affect the dependant variable of the amount of DNA extracted.
This was done to find out if primary or secondary alcohol would create more DNA precipitate than the other. For this the primary alcohols used were; methanol and ethanol, and the secondary alcohol was; isopropyl. Of this the secondary alcohol, isopropyl was discovered to be the most effect alcohol to make DNA precipitate, as it produced the most amount of DNA.
This investigation of extracting DNA is significant due to the study taken providing understanding and knowledge on DNA; this allows people to find out information of the cell structure, and what DNA does.
IntroductionThe aim of this experiment is to find the effects different types of alcohol, primary or secondary, has on the quantity of DNA extracted from strawberries.
The results should show that methanol and ethanol would have similar results due to both being primary alcohols.
Isopropyl would have better result due to it being a secondary alcohol. DNA or deoxyribonucleic acid is the nucleic acid molecule that stores the hereditary information of the organism and is indirectly responsible for cellular structure and metabolism Aubusson, Kennedy, Snyder The main role of DNA acid is the long term storage of information.
DNA is a set of blueprints or a code since it contains the instructions needed to construct other components of cells, such as proteins.
The DNA divisions that carry this genetic information are called genes; other DNA sequences have different structural purposes, for example to create a body part, like an arm or a leg. Because of this, DNA provides information on hair colour, eye colour, skin colour, etc… DNA extraction is important because it enables people to see the information about DNA and shows how complex information is stored.
DNA is found in chromosomes inside the nucleus. This DNA is wrapped around a ball shaped histone protein. It is important to achieve maximum DNA out of the results as the increased amount of DNA that is collected, the better it is able to be studied in some depth.
The reasoning behind the investigation was to study the amount of DNA extracted when using different types of alcohols. This information is needed, to know the best possible way of obtaining maximum amounts of DNA from the cells to optimise the protocol.
The different types of alcohol were methanol and ethanol primary alcohols and isopropyl secondary alcohol. There are different types of alcohols; primary, secondary and tertiary. The construction of primary, secondary and tertiary alcohols depends on the different amounts of carbon attached to the alkyl groups.
Some examples of a primary alcohol are; ethanol and methanol, secondary alcohol; isopropyl, and tertiary alcohol; tert-butyl.
DNA is not soluble in alcohol. So when alcohol is added to the mixture, mixture, except for DNA, stay in solution while the DNA precipitates out into the alcohol layer.
Also alcohol is less dense than water, so the alcohol stays on top of the mixture without mixing in. The importance of finding the most effective way of obtaining the most amount of DNA from different primary and secondary alcohols is so that it can be compared.
The detergent will act and dissolve or separate the lipid components of the cell membrane; this gives access to the proteins and nucleic acids within the cell.
The detergent cells are similar to phospholipids. The similar district of both detergent and phospholipids work together, disrupting the structure of the cell membrane and causing it to break apart.
The salt helps to break down the cell membrane by denaturing the proteins in the membrane. The enzymes in the meat tenderiser are called papain, it is used to break up the histone proteins, into smaller pieces this detaches the DNA, allowing the DNA to uncoil and be seen.
Also set up the filtering apparatus see figure 1. Next blend g of Strawberries; Measure our mLs of the strawberry mixture and mix in mLs of distilled water, then put the mixture into the filtering apparatus, and leave to filter place filtrate mixture into a beaker.
Take out the mixture and stir in 1gram of salt, 1g of meat tenderiser and 1mL of detergent. Pour 5mL of the now filtered and heated mixture into each of the 3 test tubes.
In test tube 1add 5mL of methanol gently and slowly down the inside of the test tube. In test tube 2 add 5mL of ethanol.Strawberry Fields by Miriam Wells Essay - There has been a long standing debate between the socio-economic theories of capitalism and socialism.
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